Mackie, who has scored four goals for Argyle in the Championship this term, missed Saturday's 1-0 home defeat by Sheffield United following his one-match ban for receiving five cautions.
Boss Paul Sturrock partnered World Cup-bound New Zealand international Rory Fallon in attack with recalled 20-year-old striker Ashley Barnes.
Sturrock also used the game to introduce 18-year-old forward Joe Mason.
Mason made his debut on the right of midfield while, as anticipated, fit-again central defender Kari Arnason returned in the back four and should keep his place.
Right-back David Gray - on loan from Manchester United - was also recalled and is also expected to stay in the side.
Swansea midfielder Tom Butler will miss the clash.
The Republic of Ireland international picked up a knee injury in the draw at Peterborough but is expected to be fit in time to face Nottingham Forest at the weekend.
Nathan Dyer, who came off the bench for Butler shortly after the hour mark at London Road, should replace him in the starting line-up.
Craig Beattie is the only other new injury worry and the striker faces a late fitness test.
Captain Garry Monk is still sidelined with a calf injury but fellow defender Alan Tate returns after serving his one-match suspension for picking up five bookings.
Striker Stephen Dobbie (ankle) and midfielder Mark Gower (hamstring) are also still out.
Sporting Life